Abstract
A survey was conducted to ascertain the distribution and virulence of Aeromonas spp. (species) in carabeef and mutton samples in Greater Hyderabad Municipal Corporation, India. Conventional method using Aeromonas isolation medium and Ampicillin Dextrin agar, PCR targeting 16S rRNA were used to identify Aeromonas spp. in meat samples. Conventional cultural method revealed 28% and 40% positives in mutton and carabeef, respectively whereas PCR assay detected 32% (mutton) and 44% (carabeef) positives suggesting that PCR has a better sensitivity than the cultural method. PCR positives were examined for the presence of toxin genes. Both aerolysin (37.5% in mutton and carabeef) and thermostablecytotonic enterotoxin (36.36% in mutton and 27.27% in carabeef) were detected, indicating the potential pathogenicity of Aeromonas spp. in meat products.
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