Abstract
Aim:The study was conducted to determine the prevalence and characterization of the Pasteurella multocida isolates from suspected pigs in Vietnam.Materials and Methods:A total of 83 P. multocida strains were isolated from lung samples and nasal swabs collected from pigs associated with pneumonia, progressive atrophic rhinitis, or reproductive and respiratory symptoms. Isolates were subjected to multiplex polymerase chain reaction (PCR) for capsular typing, detection of virulence-associated genes and antibiotic resistance genes by PCR. The antimicrobial sensitivity profiles of the isolates were tested by disk diffusion method.Results:All the isolates 83/83 (100%) were identified as P. multocida by PCR: serogroup A was obtained from 40/83 (48.19%), serogroup D was detected from 24/83 strains (28.91%), and serogroup B was found in 19/83 (22.35%) isolates. The presence of 14 virulence genes was reported including adhesins group (ptfA – 93.97%, pfhA – 93.97%, and fimA – 90.36%), iron acquisition (exbB – 100%, and exbD – 85.54%), hyaluronidase (pmHAS – 84.33%), and protectins (ompA – 56.62%, ompH 68.67%, and oma87 – 100%). The dermonecrotoxin toxA had low prevalence (19.28%). The antimicrobial susceptibility testing revealed that cephalexin, cefotaxime, ceftriaxone, ofloxacin, pefloxacin, ciprofloxacin, and enrofloxacin were the drugs most likely active against P. multocida while amoxicillin and tetracycline were inactive. The usage of PCR revealed that 63/83 isolates were carrying at least one of the drug resistance genes.Conclusion:Unlike other parts of the word, serotype B was prevalent among Vietnamese porcine P. multocida strains. The high antibiotic resistance detected among these isolates gives us an alert about the current state of imprudent antibiotic usage in controlling the pathogenic bacteria.
Highlights
Pasteurella multocida is a normal inhabitant of the respiratory tract of healthy animals [1]; it is an enigmatic pathogen known for being associated with a diversity of respiratory syndromes that can affect a range of host species [2]
Unlike other parts of the word, serotype B was prevalent among Vietnamese porcine P. multocida strains
The present study investigates the prevalence of virulence factor, antibiotic resistance, and capsular serotype genes of P. multocida isolated from pigs with symptoms of pasteurellosis in Vietnam
Summary
Pasteurella multocida is a normal inhabitant of the respiratory tract of healthy animals [1]; it is an enigmatic pathogen known for being associated with a diversity of respiratory syndromes that can affect a range of host species [2]. In swine, it causes progressive atrophic rhinitis (PAR) and is thought to play an important role in pneumonia [3]. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated
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