Prevalence of some virulence factor genes in bacterial acquired hospital infections isolated from hospitals of Wasit province

Abstract

Background: Antimicrobial resistance genes are an acquired phenomenon in nosocomial pathogens as results to uncontrolled and randomly used of antibiotics. Methods: A total of 308 samples were collected from different places and surfaces for bacterial contamination in three main hospitals in Wasit province. Bacteria were isolated and identified using biochemical test, then PCR was used in order to analysis he 16S rRNA gene in bacterial isolates and virulence genes using specific primers. Results: The results of bacterial cultures indicated the presence of four bacterial isolates included A. baumannii, S. marcescens, E. cloacae and S. haemolyticus, PCR product analysis of the 16S rRNA gene in bacterial isolates showed that all bacteria 100% were positive for 16S rRNA gene. PCR analysis of virulence factor AdeC gene in A. baumannii showed that 7(70%) of bacteria were positive, while 7(70%) of bacteria were positive for CsuE gene, S. Marcescens, bsmA gene were positive in 5(100%) and fimA were 0(0%) of bacteria isolates, regarding E. cloacae, fimA and CsgA genes were positive in bacterial isolates in a percentage of 5(55.5%) and 9(100%) respectively, finally in S. haemolyticus it was indicated that AtlE and Fbe genes were positive in 7(70%) and 6(50%) of bacteria isolates.