Abstract
Objective To analyze the genetic characteristics of norovirus (NoV) outbreaks in Huzhou city in 2018. Methods Totally 73 stool samples were collected from NoV outbreaks and tested by real-time RT-PCR. The polymerase and capsid protein regions were amplified from the norovirus nucleic acid positive samples in each cluster epidemic. The amplified products were further analyzed by gene sequencing and genotyping. Results A total of 57 specimens from 8 outbreaks were positive for GII type NoV nucleic acid and 5 samples were positive for both genogroup Ⅰ and genogroup Ⅱ. The alignment of polymerase and capsid protein nucleic acid sequences showed that 5 cases were GII.P16/GII.2 type, 1 case was GII.P17/GII.17 type, 1 case was GII.P12/GII.3 type, and 1 case was mixed infection with GI.P4/GI.5 type and GII.P17/GII.17 type. Conclusions NoV is the main pathogen causing the epidemic of acute gastroenteritis and diarrhea in Huzhou city. The virus was mainly GII type, with multiple genotypes and recombinant strains. Key words: Norovirus; Diarrhea; Outbreak; Prevalence
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