Prevalence of non-O157 Shiga Toxin-producing E. Coli in Children and Calves in Al- Muthanna Province, Iraq

Ahmed Jarad,Kh Al- Jeboori

doi:10.37940/ajvs.2021.14.2.10

Abstract

The present study focus on non-O157 Shiga toxin-producing E. Coli (STEC), included a bacteriological study was subjected to provide additional information for non-O157 STEC prevalence in children and calves. Isolation by using selective culturing media (CHROMagar STEC and CHROMagar O157) from 127 children suffering from diarrhea and 133 calves in Al- Muthanna province. Characterization depends on culturing positive colony on MacConkey agar and Levin’s Eosin Methylene blue agar, staining single colony from the growth by gram stain, biochemical tests; Indole, the Methyl Red, Voges-Proskauer, Citrate test, Oxidase, Catalase, Urease, Motility, Kligler Iron and Api-20E, were done to confirm a diagnosis of non-O157 STEC, The reliable isolation as non-O157 STEC serotyping by specific latex agglutination test for the target non-O157 STEC (big six) serogroup (O26, O45, O103, O111, O121 and O145). The current study showed the prevalence of non-O157 STEC was 20 of out 127 (15.73%) in samples collected from children and 27 / 133 (20.30%) in calves samples in conclusion the Non-O157 STEC is an important cause of diarrhea in children, and calves; finally, the calves play an important reservoir for Non-O157 STEC.

Highlights

The German pediatrician and scientist, Dr.Theodor Escheric isolated organism for the first time in (1885) from the intestinal flora of infants and he called them Bacterium coli commune [1,2, 3]. Later this organism was named as Escherichia coli or E. coli on his honor [4]
Bray (1945) was the first to report the link between E. coli strains and diarrhea in what was known as "summer diarrhea."The pathogenic groups includes enteropathogenic E. coli, Enterotoxigenic E.coli, diffuse adhering E. coli, enteroinvasive E. coli, enteroaggregative E.coli, Sepsis/meningitis E. coli, uropathogenic E. coli, and enterohemorrhagic E.coli or Shiga toxin-producing E. coli.(STEC)( 4, 6), Shiga toxin-producing E. Coli (STEC) are called a vero toxic E. coli (VTEC) [7]
Non-O157 STEC's importance in human diseases is less well understood than STEC O157:H7(18), and its diagnosis can be a challenge because they do not have unique or distinguishable features that make them differentiated from other strains of E. coli, so it is unlike STEC O157:H7 [19]

Summary

Introduction

The German pediatrician and scientist, Dr.Theodor Escheric isolated organism for the first time in (1885) from the intestinal flora of infants and he called them Bacterium coli commune [1,2, 3]. 1- Indol test Tubes containing peptone water were prepared and were inoculated with the colony of suspected bacteria and incubated at 37Cfor 24 hours, and several drops of Kovac’s reagent (CDH, India) prepared according to the manufacturer's instructions, were added to the broth medium without mixing, The appearance of red ring on the surface liquid of the medium was regarded as a positive result.

Results

Conclusion