Abstract
BackgroundCrohn’s disease is a chronic enteritis of humans that affects the gastrointestinal tract, especially the terminal ileum, cecum and colon. The etiology of this disease is still unknown but seems to be multifactorial. There are reports about the potential link between Crohn’s disease in humans and the causative agent of Johne’s disease in ruminants. Because of the prevalence of Johne’s disease in the Fars Province of Iran, the aim of this study was to investigate the prevalence of MAP in the biopsy tissues of patients affected by Crohn’s disease in this area.MethodsThe study was performed from April 2015 to June 2017 at Namazi Hospital, Shiraz University of Medical Sciences, and School of Veterinary Medicine, Shiraz University, Shiraz, Iran. Intestinal biopsies of 30 patients (12 male and 18 female; mean age, 34 years; range 4–77 years) with the confirmed diagnosis of Crohn’s disease and 30 patients diagnosed as non-inflammatory bowel disease (19 male and 11 female; mean age, 38 years; range 13–68 years) were studied by molecular, histopathological and histochemical methods. Also, similar numbers of adult goats affected by Johne’s disease were studied, comparatively. DNA extractions of tissue specimens were subjected to PCR to amplify a 413-bp sequence of the IS900 gene.ResultsUsing IS900-PCR, the overall prevalence of MAP in patients affected by Crohn’s disease and non-inflammatory bowel disease were 47 and 13%, respectively. In addition, the prevalence of MAP in goats affected by Johne’s disease was 70%. Using acid-fast histochemical staining, only 7% of Crohn’s disease patients were weakly positive as paucibacillary and 43% of Johne’s disease cases were moderate to strongly positive as multibacillary. Histopathologically, granulomatous enteritis (83 and 90%), lymphoplasmacytic enteritis (17 and 14%), edema and lymphangiectasia (67 and 96%), and vasculitis (20 and 73%) were common findings in Crohn’s and Johne’s diseases, respectively.ConclusionOur findings demonstrate a remarkable association between MAP and CD in this population, and support an etiologic relationship between MAP infection in humans and the development of CD. MAP infection in human tissue may display species-specific pathologic findings, as occurs with other zoonotic pathogens.
Highlights
Crohn’s disease is a chronic enteritis of humans that affects the gastrointestinal tract, especially the terminal ileum, cecum and colon
Because of the prevalence of Johne’s disease (JD) in small ruminants of the Fars province of Iran, the present study was carried out to investigate the prevalence of Mycobacterium avium subspecies paratuberculosis (MAP) in the biopsy tissues of patients affected by Crohn’s disease (CD) in this area with regard to histopathological and molecular comparison with prevalent JD
We examined paraffin embedded archival tissues of 30 patients (12 male and 18 female; mean age, 34 years; range 4–77 years) with the confirmed diagnosis of CD and 30 patients diagnosed as non-inflammatory bowel disease (19 male and 11 female; mean age, 38 years; range 13–68 years) –using molecular, histopathological and histochemical methods
Summary
Crohn’s disease is a chronic enteritis of humans that affects the gastrointestinal tract, especially the terminal ileum, cecum and colon. The etiology of this disease is still unknown but seems to be multifactorial. Because of the prevalence of Johne’s disease in the Fars Province of Iran, the aim of this study was to investigate the prevalence of MAP in the biopsy tissues of patients affected by Crohn’s disease in this area. Crohn’s disease (CD) is an idiopathic chronic regional enteritis of human that most commonly affects terminal ileum and has the potential to affect any part of the gastrointestinal tract Because of the prevalence of JD in small ruminants of the Fars province of Iran, the present study was carried out to investigate the prevalence of MAP in the biopsy tissues of patients affected by CD in this area with regard to histopathological and molecular comparison with prevalent JD
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