Abstract
The aims of this study were to evaluate the incidence of mutans streptococci (MS - sessile form) on complete maxillary dentures after use of a specific denture paste, and to determine the minimum inhibitory concentration (MIC) and maximum inhibitory dilution (MID) of 3 oral mouthrinses: Cepacol, Plax and Periogard. Seventy-seven complete denture wearers were randomly assigned into 2 groups, according to the product used for denture cleaning: Control group - conventional dentifrice (Kolynos-Super White); and Test group: experimental denture cleaning paste. Denture biofilm was collected at baseline and after 90 and 180 days after treatment by brushing the dentures with saline solution. After decimal serial dilution, samples were seeded onto agar sucrose bacitracin to count colonies with morphological characteristics of MS. MS identification was performed by the sugar fermentation tests. After this procedure, brain heart infusion broth (BHI) was added to oral mouthrinses (Plax, Cepacol e Periogard) and seeded on Petri dishes. The colonies were seeded using the Steers multiplier and, after the incubation, the MIC and MID of the mouthrinses were calculated. The results showed an incidence of 74.0% (n=57) of MS in the 77 complete dentures examined in the study, being 76.3% (n=29) of the Control group (conventional dentifrice) and 71.8% (28) of the Test group (experimental denture cleaning paste). In both groups, the number of positive cases for MS decreased from day 0 to day 180. In the Test group there was a slight decrease in the incidence of Streptococcus mutans 90 days after use of the experimental denture cleaning paste, which was not observed in the Control group. As regards to mouthrinses, for both groups, Periogard showed antimicrobial action with the highest dilution, followed by Cepacol and Plax. In conclusion, the incidence of MS in complete dentures was high and Periogard was the mouthrinse with the strongest antimicrobial action against MS. The experimental denture cleaning paste showed a slight action against S. mutans after 90 days of treatment.
Highlights
The oral health of elderly denture wearers is usually inadequate, and lesions associated with poor hygiene are a common occurrence [1,2]
Biofilm is a dense microbial layer formed by microorganisms and their metabolites [5]
Patients were distributed into two groups, according to the treatment protocols: Control Group (n=38): subjects were provided with a conventional toothpaste recommended for cleaning natural teeth (Kolynos Super White; Kolynos do Brazil Ltda., São Bernardo do Campo, SP, Brazil); Test Group (n=39): subjects were given an experimental denture cleaning paste [13,15,16]
Summary
The oral health of elderly denture wearers is usually inadequate, and lesions associated with poor hygiene are a common occurrence [1,2]. This aspect emphasizes the denture wearers need to be aware of the care required for functional maintenance of the denture. Biofilm is a dense microbial layer formed by microorganisms and their metabolites [5]. Mutans streptococci (MS) are microorganisms of great importance in biofilm formation. These microorganisms produce polysaccharides from sugar metabolism that have favor their adhesion and that of yeasts to the oral mucosa and denture surfaces [6]
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