Prevalence of Listeria monocytogenes in Raw Cow's Milk in the Region of Batna, Algeria

Sabrina Rabehi,Asma Meghezzi,Taha Mossadak Hamdi,Bakir Mamache,Khaoula Boushaba

doi:10.3844/ajavsp.2021.212.216

Sabrina Rabehi, Asma Meghezzi + Show 3 more

Open Access

https://doi.org/10.3844/ajavsp.2021.212.216

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Abstract

The aim of this study is the detection of the presence of L. monocytogenes in raw milk in the region of Batna using the real-time polymerase chain reaction (real-time PCR) technique, in order to assess the riskrun by the consumer of raw milk in the study area. 65 milk samples were investigated for the detection of L. monocytogenes. The detection of the hlyA gene in all samples was done by real-time PCR assay by comparative cycle threshold method. The results obtained were negative for all the samples analyzed, therefore the prevalence of L. monocytogenes in the 65 samples collected in this study is 0%. The absence of L. monocytogenes in the samples could be linked to the good health of the selected cattle. In fact, L. monocytogenes is considered to be very widespread in industrialized countries and rare in North Africa and Algeria.

Highlights

L. monocytogenes is a foodborne pathogen responsible for listeriosis in humans due to its presence in raw and ready-to-eat foods which are stored at refrigeration temperature (Shi et al, 2015)
Contaminated food with Listeria monocytogenes is the predominant route of transmission of listeriosis to humans, a severe illness with a high mortality rate (Magalhães et al, 2017)
The results obtained were negative for all the samples analyzed, the prevalence of L. monocytogenes in the 65 samples collected in this study is 0%

Summary

Introduction

L. monocytogenes is a foodborne pathogen responsible for listeriosis in humans due to its presence in raw and ready-to-eat foods which are stored at refrigeration temperature (Shi et al, 2015). The main routes of contamination for animals (especially livestock) are silage (Welchman et al, 1997) and the animals themselves. This animal carriage of L. monocytogenes in the agricultural environment can lead to contamination of animal products such as raw milk (Huntet al., 2012) or meat (Dmowska et al, 2013). Contaminated food with Listeria monocytogenes is the predominant route of transmission of listeriosis to humans, a severe illness with a high mortality rate (Magalhães et al, 2017). Milk constitutes one of the main vectors of Listeria transmission; more raw milk from cows due to its wide consumption

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