Prevalence of Klebsiella pneumoniae in surgical practice and laboratory dog husbandry room environments at Rajamangala University of Technology Tawan-ok Veterinary Hospital

Kulchai Nakbubpa,Thitichaya Chukiatsiri,Krittamet Rommaneeyachitto,Kunyavee Phattanakitjumroon,Visuda Jivarangsan,Suchawan Pornsukarom

doi:10.12982/vis.2021.024

Kulchai Nakbubpa, Thitichaya Chukiatsiri + Show 4 more

Open Access

https://doi.org/10.12982/vis.2021.024

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Abstract

Klebsiella pneumoniae is commonly found in environments, causing secondary infections in both human and animals, as well as antibiotic resistance problem. The objective of this study was to determine the prevalence of K. pneumoniae contaminated in the environments of surgical practice and laboratory dog husbandry rooms at Animal hospital, Rajamangala University of Technology Tawan-ok during 2019 - 2020. Two-hundred-swabbed samples were collected from these nearby environments and laboratory dogs themselves. Then, all samples were tested for K. pneumoniae and antibiotic susceptibility using disk diffusion method. From the results, the total prevalence was 3.5% and the high frequencies were found in three categories: laboratory dogs (35.7%), veterinarian belongings (3.3%) and building structure (2.5%). The antibiotic resistance was also detected including Cephalexin, Ceftazidime and Penicillin G (100%), Gentamicin and Colistin (85.71%), Amikacin (42.86%), and Ciprofloxacin (14.29%). Owning to the fact that laboratory dogs exposed to an external environment leading to bacterial contamination into themself and the husbandry room. Therefore, the hygiene of laboratory dogs and area in husbandry room should be primarily considered. Our study would be the preliminary baseline for the study of antibiotic resistant K. pneumoniae contamination in dogs, human, and their environments.

Highlights

Klebsiella is gram negative bacilli in family Enterobacteriaceae, non-motile with encapsulated rod shape (Podschun and Ullmann, 1998)
From all 200 samples swabbed from surgical practice and trial dog husbandry rooms at Animal hospital, the overall prevalence of K. pneumoniae was 3.5% (7/200)
In accordance with our study, we found the prevalence of this bacteria in building structures especially sewer pipes as 10% (1/10) (Table 1), correlated to the data documented by Wareth and Neubauer (2021) that K. pneumoniae was found in raw waste water and water specimens

Summary

Introduction

Klebsiella is gram negative bacilli in family Enterobacteriaceae, non-motile with encapsulated rod shape (Podschun and Ullmann, 1998). Klebsiella spp. are ubiquitous in gastrointestinal tract of various animal species including domestic and wild mammals as well as insects and have been recovered from foods (Guo et al, 2016). They can be found in certain environments such as soil, dust, surface waters, and plants (Seidler et al, 1975; Bagley et al, 1978). K. pneumoniae is considered to be one of the most important species among genus Klebsiella causing virulent infection, secondary infection, nosocomial infection and various diseases in human and animals. There was a report of Klebsiella spp. infection in a bull terrier dog’s hard palate after surgical repair of cleft hard palate (Stevanovic et al, 2019)

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