Prevalence of IgG Antibodies and Cytomegalovirus DNA on blood donor at Blood Transfusion Unit of DKI Jakarta Province

Abstract

To date, the seropositive prevalence of CMV in blood donor is still remaining unknown. Therefore, no screening test for CMV antibody and CMV DNA analysis on leukodepleted PRC that is routinely performed in Indonesia for the safety of the blood donor. The purpose of this study was to obtain information on the effectiveness of PRC leukodepleted techniques on CMV DNA detection, to obtain information on the prevalence of PRC with positive CMV IgG antibodies and to obtain information on the prevalence of positive CMV DNA in non-leukodepleted PRC and leukodepleted PRC at UTD PMI DKI Jakarta . Cross sectional design with total sample of 113 donor blood that has fulfilled the inclusion criteria was used as methodology. Indirect chemiluminescence immunoassay (ChLIA) method with Liason® XL 10050 Chemiluminescence Analyzer was used for IgG CMV antibody screening test and qPCR technique with UL54 CMV by Roche light cycler 480 II was used for CMV DNA analysis. The results showed that 111 samples (98.23%) were positive to IgG CMV and 2 samples (1.77%) was negative to CMV IgG. The prevalence of positive CMV DNA in PRC before leukodepleted was 1 sample (0.88%) and PRC after leukodepleted was 0 sample (0%). The conclusion of this study is leukodepleted PRC effective to reduce the spread of CMV infection through blood transfusions. Key words: Cytomegalovirus, PRC Leukodepleted, IgG CMV, qPCR UL54 CMV.