Abstract
JC virus (JCV) , and BK virus (BKV) can remain latency in kidney and excrete via urine asymptomatically. JCV has been associated with colorectal and bladder cancers. BKV has been linked with lung, pancreas, liver, urogenital tract, head and neck cancers. Therefore, the frequency of JCV DNA and BKV DNA are essential to evaluate in urine samples of healthy individuals. Materials and Methods:Hundred sixty four urine samples were collected from healthy subjects [96 females and 68 males]. DNA was extracted and detection of JCV DNA and BKV DNA was carried out by PCR . The analysis of sequencing and construction of phylogenetic tree were performed for the samples positive for JCV DNA and BKV DNA. Results:Ten (6.09%) urine samples [5/96(5.2%) females and 5/68( 8.82) males] were tested positive for JCV DNA (P= 0.814). The results of sequencing and phylogenetic tree showed the isolated JCV DNA were cluster with 3A genotype. 21/164 (12.8%) samples were tested positive for BKV DNA [11/96(11.45%) females and males 10/68(14.7%)] ( P= 0.63). The results of sequencing and phylogenetic tree showed that the isolated BKV was cluster with genotype III. Conclusion:In the present study 6.09% and 12.8% of the healthy individuals showed positive for JCV DNA (genotype 3A) and BKV DNA(genotype III) respectively. With regard to life threating diseases by BKV and JCV in immunocomprsied patients , the screening BKV DNA and JCV DNA should be implemented for patients with cancer /autoimmune diseases /organ recipient/ multiple sclerosis (MS), prior to immunosuppression therapy or immunomodulatory agents treatment.
Highlights
JC virus (JCV) or John Cunningham virus (JCPyV) and BK Polyomavirus (BKPyV) are belong to polyomaviridae family (DeCaprioet al.,2013)
The results of sequencing and phylogenetic tree showed the isolated JCV DNA were cluster with 3A genotype. 21/164 (12.8%) samples were tested positive for BK virus (BKV) DNA [11/96(11.45%) females and males 10/68(14.7%)] ( P= 0.63)
The results of this study showed that 10/ 164(6.09%) samples [5/96(5.2%) females and 5/68( 7.35% ) males] were tested positive for JCV DNA (P= 0.814)
Summary
JCV or John Cunningham virus (JCPyV) and BK Polyomavirus (BKPyV) are belong to polyomaviridae family (DeCaprioet al.,2013). JCV and BKV are prevalent in human populations worldwide (Moens et al.,2017, Boothpur et al, 2010). BKV virus has been detected in urine sample of 10%-60% of the kidney transplant patients (Garces et al, 2010) Both the viruses share same rout of transmission and are transmitted via respiratory or oral-fecal rout (Bofill-Mas et al, 2001). Based on hypervariable region of VP1, the JCV has been classified into 7 genotypes (DeCaprioet al., 2013; Moens et al, 2017; Boothpur et al, 2010; Nali et al, 2012; Jeong et al, 2004; Garces et al, 2010; BofillMas et al, 2001). Genotypes II and III are rare (~ 5%) in all geographic regions (Morel et al, 2017)
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