Abstract
ObjectiveThe aim of this study was to analyze the prevalence of HPV and EBV in oral and oropharyngeal squamous cell carcinoma in south-eastern Poland. The correlation between viral infection, OSCC, alcohol use, tobacco smoking, demographic data (gender, age, place of residence), anatomic location, pre-treatment staging, evidence of metastases to lymph nodes, and grading was also investigated.MethodsThe examination samples were collected from paraffin tissue blocks, from 154 patients. Viral DNA was amplified by the nested-PCR method.ResultsHPV DNA was detected in 29.2 % of the tested samples (in 27.4 % of oropharyngeal and in 30.4 % of oral cavity). The HPV type 16 was detected in 15.6 % of all samples, and in 53.3 % of HPV-positive group. In HPV-positive samples from oropharyngeal HPV 16 constitute 76.5 %, and in HPV-positive samples from oral cavity HPV 16 constitute 39.3 %. Mixed infection (more than one type of HPV) was observed in 23.5 and 60.7 %, respectively, and in 46.7 % of all HPV-positive samples, and in 12.3 % of the whole study group. EBV DNA was detected in 27.3 % of the cases and HPV-EBV co-infection in 7.8 % of samples.ConclusionsIn major patients from Southeastern region of Poland with oropharyngeal cancer HPV type 16 was detected but in oral cavity cancer other mixed infections were observed (i.e. 51, 52, 59, 66, 68, 71, 74). HPV 16 was detected more often among patients younger than 50 years of age, whereas the mixed HPV in the group aged 50–59. The pathogenesis of oral squamous cell carcinoma may be connected with EBV infection. Future studies on the mechanisms of HPV/EBV co-infection and/or superinfection and their role in oral squamous cell carcinoma are necessary.
Highlights
Oral squamous cell carcinoma (OSCC) is a serious public health problem in many parts of the world and in Poland too
The first original observation that implicated HPV as a risk factor in the development of oral cancer was presented by Syrjänen et al in 1983 [6]
A more recent study by Syrjänen and Syrjänen showed a strong association between the presence of HPV DNA, Polz-Gruszka et al Infectious Agents and Cancer (2015) 10:37 HPV16, and oropharyngeal squamous cell carcinoma (OSCC) [8]
Summary
Oral squamous cell carcinoma (OSCC) is a serious public health problem in many parts of the world and in Poland too. Oral and oropharyngeal squamous cell carcinoma (OSCC) in tumor histology accounts for more than 90 % of the cases [2]. A more recent study by Syrjänen and Syrjänen showed a strong association between the presence of HPV DNA, Polz-Gruszka et al Infectious Agents and Cancer (2015) 10:37 HPV16, and OSCC [8]. This meta-analysis showed that HPV significantly increases the risk for OSCC, as compared with controls. HPV positive oropharyngeal cancer varied according to the geographical region, i.e. in North America – 56 %, in Japan 52 %, in Australia 45 %, in Northern and West Europe 38 % [9]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
