Abstract
Abstract Objective To investigate the prevalence of Helicobacter pylori ( H. pylori ) infection in dyspepsia patients and its relation to virulence factor cagA gene. Methods In total, 110 gastric biopsies from dyspeptic patients were comparatively studied using rapid urease test and multiplex PCR. Multiplex PCR detected three genes of 16S rRNA, cagA, and ureC. Results H. pylori was detected in 14 gastric biopsies (13%). Significantly higher number of female were infected. Furthermore, cagA gene was found in all H. pylori –positive specimens. In addition, the result indicated that the multiplex PCR with annealing temperature at 57 oC was able to effectively amplify specific products. Conclusion The results confirmed that high preva¬lence of cagA gene in H. pylori among dyspeptic patients in Southern Thailand.
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