Abstract
Enterotoxigenic Escherichia coli is a major cause of diarrhea in neonatal and post-weaning piglets. To determine the most common fimbrial antigens of ETEC in piglets with diarrhea, two investigations were carried out on intensive pig farms in Hubei province, central China. In 2002-2003, 227 fecal samples from neonatal and post-weaning piglets with diarrhea were tested for the presence of the fimbrial antigen K88 and K99 of ETEC by the polymerase chain reaction (PCR). Twenty-three (10.1%) of 227 fecal samples were found to contain fimbrial antigen K88, which was identified as K88ac variant; and 13 (5.7%) samples containing K99. In 2004, another 179 fecal samples from diarrheic piglets, 1 day to 6 weeks of age, were tested for prevalence of fimbrial antigen K88, K99 and 987P. Forty-seven (26.3%) of the 179 samples carried at least one of the ETEC fimbrial antigens. K88 antigen was detected in 20.1%. In the 36 samples known to carry fimbrial antigen K88, 32 (88.9%) contained K88ad; and 4 (11.1%) contained K88ac; none of them carried K88ab. Fimbrial antigens K99 and 987P were detected in 1.1% and 6.1%, respectively. Our data indicate that K88 is the most common fimbrial antigen of ETEC associated with diarrhea in piglets in Central China.
Highlights
Enterotoxigenic Escherichia coli is a major cause of diarrhea in neonatal and post-weaning piglets
K88 receptors in (HI) and immunoserology reaction, such as the serum the brush border are independent of age, so ETEC that agglutination test (SAT), indirect fluorescent antibody test (IFAT) and enzyme-linked immunosorbent assay (ELISA)
Investigating fimbrial antigen characteristics of ETEC strains by polymerase chain reaction (PCR) using specific primers does not require high purity of sample (Franklin et al, 1996)
Summary
Enterotoxigenic Escherichia coli is a major cause of diarrhea in neonatal and post-weaning piglets. To determine the most common fimbrial antigens of ETEC in piglets with diarrhea, two investigations were carried out on intensive pig farms in Hubei province, central China. In 2002-2003, 227 fecal samples from neonatal and post-weaning piglets with diarrhea were tested for the presence of the fimbrial antigen K88 and K99 of ETEC by the polymerase chain reaction (PCR). (Key Words : Enterotoxigenic Escherichia Coil, Fimbrial Antigen, PCR, Piglets, Diarrhea). Enterotoxigenic Escherichia coliďETECĐis a major intestinal disease Using therapeutic antibodies, such as egg cause of diarrhea and death in neonatal and post-weaning yolk antibodies to anti-frimbrial antigens, was reported as piglets
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