PREVALENCE OF EXTENDED SPECTRUM ß-LACTAMASE PRODUCING E. COLI ISOLATED FROM IN-PATIENTS AND OUT-PATIENTS IN ZAGAZIG UNIVERSITY HOSPITALS

Abstract

This study was done on 143 patients (75 in-patients and 68 out-patients) in Zagazig University Hospitals who were suspected to have urinary tract infections (UTIs). Their ages ranged from 12 to 61years. One hundred and twenty four of urine samples that collected from patients yielded positive culture on MacConkey’s agar. The identification of isolates was done by biochemical tests and confirmed by automated Vitek 2 system. The results showed that E. coli was the most causative organisms of UTIs 44 isolates (35.4%). Antimicrobial susceptibility test by modified Kirby-Bauer disc diffusion method was performed to select multi drugs resistant (ESβL) production according to the Clinical and Laboratory Standards Institute (CLSI) recommendation. The PCR technique was applied to detect the ESβL genes that responsible for the resistance, it was found that the TEM gene was the predominate gene (100%) amongst ESβL E. coli followed by SHV (90%) but CTX-M gene was absent. The coexistence of more than one type of β-lactamase enzymes was detected and represented (30%) of ESβL E. coli. From these results we can concluded that the Prevalence of ESβL producing E. coli was (22.7%) among E. coli isolates. TEM and SHV genes were the most frequent genes and the coexistence of ESβL, AmpC, OXA-1 was detected in (30%) of isolates.Keywords: ESBL, UTI,