Abstract
In this study, 194 samples of the dairy supply chain from various regions were collected, and the most prevalent pathogen discovered was Enterococcus spp., which was present at a rate of 25.77% (50/194), followed by S. aureus and E. coli, which were present at rates of 15.97% (31/194) and 14.94% (29/194), respectively. The double disc approach was used in this investigation to phenotypically confirm that five different E. coli isolates were Extended spectrum β-lactamases (ESBL) producers. By phenotyping by disc diffusion testing, it was determined that five isolates of S. aureus with coagulase positive were methicillin resistant (MRSA), while one sample of Enterococcus was found to be vancomycin resistant. This was confirmed using the BD Phoenix Diagnostic System. Further, three salmonella isolates found through conventional based techniques were confirmed as Citrobacter freuendii (n = 2), Serratia marcescens (n = 1), and Mannhemia histolytica (n = 1) using the Phoenix approach.
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