Abstract
Erysipelothrix species were isolated from the tonsils of 79 (6.4%) of 1, 236 healthy cattle slaughtered in 4 prefectures, Yamagata, Miyagi, Nagasaki, and Tokyo, in Japan from September, 1998, to August, 1999.A combination, modified culture medium consisted of brain heart infusion (BHI) broth containing 0.1% Tween 80, 5% horse serum, 50μg/ml gentamicin, 0.1% sodium azide and 0.001% crystal violet, and BHI agar containing 0.1% Tween 80, 50μg/ml gentamicin, and 0.1% sodium azide was compared with two traditional media for isolation of Erysipelothrix species. The modified media were the most effective since they produced the highest number of Erysipelothrix isolates (p<0.01) as well as the greatest degree of inhibition of the growth of contaminated organisms.The isolation rates in summer (10.3%) and spring (7.1%) was higher than those in autumn (2.6%) or winter (1.3%) (p<0.01) .
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