Abstract
This study aimed to estimate the prevalence of enterotoxigenic Escherichia coli (ETEC) and Shiga toxin-producing Escherichia coli (STEC) strains in pigs slaughtered in abattoirs located in the state of Mato Grosso, Brazil. Intestinal samples from 74 animals were aseptically dissected and lumen content was plated on MacConkey agar. Confluent colonies from each plate were screened for the presence of ETEC and STEC strains by PCR assays. It was verified that the prevalence of STEC and ETEC carriers was 1.35% and 9.46% respectively. One (1.35%) of the 74 samples tested was positive for the stx2 gene, and seven (9.46%) for st1, of which two (2.70%) were also positive for lt1. The results provided represent a benchmark for future research on pathogenic E. coli of porcine origin in Mato Grosso.
Highlights
This study aimed to estimate the prevalence of enterotoxigenic Escherichia coli (ETEC) and Shiga toxin-producing Escherichia coli (STEC) strains in pigs slaughtered in abattoirs located in the state of Mato Grosso, Brazil
To estimate the prevalence of ETEC and STEC carriers, a statistically adequate sample size was calculated on the basis of the number of 800,000 pigs slaughtered per year, an expected prevalence of 5%, a 95% confidence level and an accuracy of 5% using Win Episcope 2.0 (Universidad de Zaragoza, Zaragoza, Spain)
It is well established that ETEC from animals normally do not infect humans, since these strains harbour colonization factors with remarkable host specificity [2]
Summary
This study aimed to estimate the prevalence of enterotoxigenic Escherichia coli (ETEC) and Shiga toxin-producing Escherichia coli (STEC) strains in pigs slaughtered in abattoirs located in the state of Mato Grosso, Brazil. Conclusion: The results provided represent a benchmark for future research on pathogenic E. coli of porcine origin in Mato Grosso. Enterotoxigenic E. coli (ETEC) is considered a major cause of infantile diarrhoea in developing countries and is frequently associated with traveller’s diarrhoea [4]. ETEC is a major cause of postweaning diarrhoea [5]. This E. coli pathotype causes diarrhoea by adhering to intestinal mucosa through its unique colonization factors and producing either heat-labile enterotoxins (LT-I and LT-II), heatstable enterotoxins (STa and STb), or both [4]. Due to the species-specific binding of the fimbrial adhesins, ETEC is not regarded as a zoonotic agent [2]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
