Prevalence of E.Coli in broiler chickens in winter and summer seasons by application of PCR with its antibiogram pattern

Abstract

A total of 205 chicken samples from apparently healthy broiler chickens (35 and 30), diseased broilerchickens (35 and 30) and freshly dead ones (35 and 40) were collected in winter (from December toFebruary) and summer (from June to August) seasons, respectively from Menofyiea government. Theresults showed that the incidence of E.coli in apparently healthy broiler chickens was 15.7%, diseasedbroiler chickens 37.1% and in freshly dead ones 55% in winter season while in summer season was15.8% in apparently healthy, 17.5% in diseased broiler chickens and 18.7% in freshly dead one. Theserogroups of E.coli that obtained by serological identification were O128, O78, O111, O124, O55, O142, O114,O2 and O1. The results of antibiotic sensitivity test for isolated E.coli showed that the isolated E.coliwere highly sensitive for norfloxacin (60%), gentamycin(50%) , neomycin (50%) , streptomycin(50%)and chloramphenicol (50%). moderately sensitive for doxcyclin (10%) and erythromycin(40%) andhighly resistant for amoxacillin /clavulinic acid (0%). The results of multiplex PCR showed that eae A(intimin or E.coli attaching and effacing) gene detected in O128,O55, O1and O2, OmpA (outer membraneprotein ) gene detected in all E.coli serogroups that isolated , stx1 gene not detected in all E. colisergroups that isolated ,but stx2 gene detected in O114 and O128.