Prevalence of Cryptosporidium in small ruminants from Veracruz, Mexico.

Dora Romero-Salas,José O Merino-Charrez,Mariel Aguilar-Domínguez,Nelly Ibarra-Priego,Jesús Hernández-Tinoco,Cosme Alvarado-Esquivel,Adalberto A Pérez De León,Anabel Cruz-Romero

doi:10.1186/s12917-016-0638-3

Abstract

BackgroundCryptosporidiosis is a zoonotic disease caused by the protozoan parasite Cryptosporidium spp. that can affect domestic animal and human populations. In newborn ruminants, cryptosporidiosis is characterized by outbreaks of diarrhea, which can result in high morbidity and economic impact. The aim of the present study was to determine the prevalence of Cryptosporidium spp. in small ruminants from the Perote municipality in Veracruz State, Mexico. One hundred and sixty small ruminants (80 sheep and 80 goats) from eight farms located in four towns of the Perote municipality were examined following a cross-sectional study design. Stool samples were analyzed by a modification of the Faust centrifugation method, and the presence of Cryptosporidium spp. oocysts was examined using a modification of the Ziehl-Neelsen staining procedure. Bivariate and multivariate analyses were used to assess the association of Cryptosporidium infection and the general characteristics of the animals studied.ResultsOverall, 112 (70 %, 95 % CI: 62.3–76.9) of the 160 small ruminants sampled were infected with Cryptosporidium spp. The prevalence of Cryptosporidium spp. infection in goats was 72.5 % (95 % CI: 61.4–81.9) and in sheep 67.5 % (95 % CI: 56.1–77.6). Small ruminants aged 1 month old had the highest (88.2 %; 95 % CI: 63.6–98.5) prevalence of infection. Prevalence varied from 60 % to 85 % among herds. Animal species, age, sex, breed, farm, town or cohabitation with cattle did not influence the prevalence of Cryptosporidium infection.ConclusionsA high prevalence of infection with Cryptosporidium spp. was observed in small ruminants from the Perote municipality in Veracruz, Mexico. Infection was widely distributed among sheep and goats regardless of their age, breed or farm location. Further research is required to identify risk factors for, and to assess the veterinary public health significance of Cryptosporidium infection among sheep and goats in the Mexican state of Veracruz.

Highlights

Cryptosporidiosis is a zoonotic disease caused by the protozoan parasite Cryptosporidium spp. that can affect domestic animal and human populations
Very little is known about the infection with Cryptosporidium spp. in sheep and goats in Mexico in general and there is a lack of information about this infection in the southern Mexican state of Veracruz in particular
Whereas of the 80 goats studied, 58 (72.5 %; 95 % confidence interval (CI) 61.4–81.9 %) were positive for Cryptosporidium infection. These results provide for the first time evidence of Cryptosporidium spp. infection in sheep and goats in the municipality of Perote in Veracruz, Mexico

Summary

Introduction

Cryptosporidiosis is a zoonotic disease caused by the protozoan parasite Cryptosporidium spp. that can affect domestic animal and human populations. The aim of the present study was to determine the prevalence of Cryptosporidium spp. in small ruminants from the Perote municipality in Veracruz State, Mexico. Infection with Cryptosporidium in young sheep and goats may lead to morbidity and even mortality due to diarrhea [20,21,22]. Effective disease prevention requires an understanding of the environmental factors predisposing animal and human populations to infectious causes of diarrhea like Cryptosporidium spp. We sought to determine the prevalence of infection with Cryptosporidium spp. in sheep and goats in the municipality of Perote in Veracruz State, Mexico

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