Abstract
BackgroundK. pneumoniae is a gram-negative pathogen that common cause of nosocomial infections. The rapid spread of multi-drug resistant (MDR) K. pneumoniae is an important issue. This study aimed to identify carbapenemases (Klebsiella pneumoniae carbapenemase [KPC], oxacillinase- 48 [OXA-48], New Delhi metallo-β-lactamase-1 [NDM-1]), oxacillinase-181 [OXA-181] and extended-spectrum beta-lactamases (ESBL) (CTX-M-15) were identified among K. pneumoniae isolates in Ahvaz, Iran. MethodsIn this cross-sectional study, conducted from July 2018 to July 2019, isolates of K. pneumoniae obtained from various clinical samples. All Gram-negative bacilli isolates were differentiated on species level by standard biochemical tests. In following, antibiotic susceptibility testing by disk diffusion method, ESBLs, carbapenemase, metallo-beta-lactamase and blaCTX-M-15, blaKPC, blaOXA-48, blaNDM, and blaOXA-181 genes were performed. ResultsIn total, 120 clinical isolates of K. pneumoniae were obtained. The most effective antibiotics for K. pneumoniae isolates were colistin, imipenem, and doripenem, with 98.3%, 64.1%, and 64.1% susceptibility. Thirty-one isolates (25%) were resistant to meropenem disc and considered as carbapenem-resistant. A total of 78 isolates (65%) were ESBLs by double-disc synergy test (DDST) and 25 isolates (20.8%) produced metallo-beta-lactamase. Among 31 (25%) carbapenem-resistant isolates, 22 isolates (71%) hydrolyzed the meropenem and classified as carbapenemase producers. PCR assays revealed that prevalence of blaCTX-M-15, blaKPC, blaOXA-48, blaNDM, and blaOXA-181 genes was 36.7%, 10%, 64.15%, 1.7% and 8.3%, respectively. ConclusionThis study found a high prevalence of MDR K. pneumoniae harboring MBL and ESBL genes. It is recommended that local periodic. Surveys are performed for controlling antimicrobial resistance.
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