Prevalence of Calicivirus and Hepatitis A virus in bivalve molluscs from Galicia

Abstract

Event Abstract Back to Event Prevalence of Calicivirus and Hepatitis A virus in bivalve molluscs from Galicia David Polo1, Miguel F. Varela1, Cristina Álvarez2 and Jesus L. Romalde1* 1 Universidad de Santiago de Compostela, Microbiologia y Parasitologia, Spain 2 INSTITUTO TECNOLÓXICO PARA O CONTROL DO MEDIO MARIÑO DE GALICIA, Microbiologia, Spain From a virological standpoint, shellfish safety continues to be a sanitary challenge. Bivalves are one of the most common vehicles of viral illness and the adoption of viral standards into European Union legislation is being considered. In this study, a 18-months survey were conducted in ten harvesting areas from two estuaries in Galicia (NW of Spain), the most important bivalve production area in Europe. Hepatitis A virus (HAV) and human norovirus (NoV), including genogroups I (GI) and II (GII) were quantified by reverse transcription-real time PCR (RT-qPCR), according to the recently developed standard method ISO/TS 15216:2013. Four bivalve species were studied, including wild and cultured mussels (Mytilus galloprovincialis), clams (Venerupis philippinarum and V. decussata) and cockles (Cerastoderma edule). Overall, 54.8% of the analysed samples were contaminated by at least one of the studied viruses, being detected the simultaneous presence of two or three viruses in 11.3% of the samples. NoV GI was the most prevalent virus (32.1%) followed by NoV GII (25.6%) and HAV (9.5%). The results showed the presence of viral contamination throughout the year in both areas and all species of molluscs. However, diverse patterns of prevalence and seasonality were observed among different viruses. HAV was detected only between March and June 2012. NoV GI was detected intermittently throughout the study period, with significant peaks in the spring and summer of 2011. NoV GII showed a clear seasonality to during the cold months by 2011; however, a significant peak was detected in the spring of 2012. Keywords: Hepatitis A virus, Norovirus, molluscs, Prevalence, RT-qPCR Conference: IMMR | International Meeting on Marine Research 2014, Peniche, Portugal, 10 Jul - 11 Jul, 2014. Presentation Type: Oral Presentation Topic: SEAFOOD TECHNOLOGY Citation: Polo D, Varela MF, Álvarez C and Romalde JL (2014). Prevalence of Calicivirus and Hepatitis A virus in bivalve molluscs from Galicia. Front. Mar. Sci. Conference Abstract: IMMR | International Meeting on Marine Research 2014. doi: 10.3389/conf.fmars.2014.02.00170 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 25 Apr 2014; Published Online: 18 Jul 2014. * Correspondence: Dr. Jesus L Romalde, Universidad de Santiago de Compostela, Microbiologia y Parasitologia, Santiago de Compostela, 15782, Spain, jesus.romalde@usc.es Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers David Polo Miguel F Varela Cristina Álvarez Jesus L Romalde Google David Polo Miguel F Varela Cristina Álvarez Jesus L Romalde Google Scholar David Polo Miguel F Varela Cristina Álvarez Jesus L Romalde PubMed David Polo Miguel F Varela Cristina Álvarez Jesus L Romalde Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.