Abstract
Buxtonella sulcata multiplication in lumen of the intestinal tract lead to Diarrhea. A total of 120 sheep faecal samples were collected (23 males and 97 females) with ages ranged from less than 1 year to 3 years. Also, 11 sheep drinking (river) water from Al-Suwaira city, Wasit governorate, for the period extended from September 2015 to the end February 2016. Laboratory methods used were direct wet film and iodine and Giemsa staining to detect the cysts and trophozoite of the parasite . Results recorded (30.83%) infection rate of Buxtonella sulcate in sheep, while the contamination rate of water (45.45%). Females are infected more than males which reach (35.13%) (13.04%) respectively. The highest infection rate (44.00%) showed in age group 2-3 years, while, the lowest (21.42%) found in age group (˃ 2). %), with significant difference (p<0.05) between ages and genus. Our conclusion is contamination of water acts as a good sourse for the spread of infection with Buxtonella sulcate .
Highlights
Buxtonella sulcata is a commensal protozoan of the alimentary tract of ruminants [1]
Made direct wet film by Place a drop of sample on the glass slide and cover with a cover slide and Giemsa staining were used After the sample has been prepared with methanol to detect cyst and trophozoite of B. sulcata in both faecal and drinking water samples under power magnification (40X and 100X) (10 and 11)
Microscopical identification of Direct smears from fecal and water samples or smears stained with Giemsa and iodin stain revealed B. sulcata trophozoites as oval body entirely covered by clear long cilia with a kidney shape macronucleus and a smaller micronucleus
Summary
Buxtonella sulcata multiplication in lumen of the intestinal tract lead to Diarrhea. A total of 120 sheep faecal samples were collected (23 males and 97 females) with ages ranged from less than 1 year to 3 years. 11 sheep drinking (river) water from Al-Suwaira city, Wasit governorate, for the period extended from September 2015 to the end February 2016. Results recorded (30.83%) infection rate of Buxtonella sulcate in sheep, while the contamination rate of water (45.45%). Females are infected more than males which reach (35.13%) (13.04%) respectively. The highest infection rate (44.00%) showed in age group 2-3 years, while, the lowest (21.42%) found in age group (˃ 2). %), with significant difference (p
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