Prevalence of Burkholderia glumae in rice crops in Ecuador

Lenin Celiano Paz-Carrasco,Roberto E Celi-Herán,Luz Diana Intriago-Mendoza,Marcos Fernando Basso

doi:10.46429/jaupr.v102i1-2.17532

Lenin Celiano Paz-Carrasco, Roberto E Celi-Herán + Show 2 more

Open Access

https://doi.org/10.46429/jaupr.v102i1-2.17532

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Abstract

Burkholderia glumae is the agent responsible for bacterial panicle blight disease (BPBD) of rice that causes severe damage to this crop worldwide. During 2012 and 2013, symptoms of BPBD were observed in Palestina city, located in Guayas province, Ecuador. In 2014, the presence of B. glumae was confirmed at this location. In view of the socio-economic importance of rice in Ecuador, this research aimed to investigate the prevalence of B. glumae in other rice-producing regions. Eighteen bacterial isolates obtained from blighted kernels were characterized. Physiological, biochemical, serological, and molecular assays and the amplification of the 16S-23S rRNA ITS of the bacterial isolates collected confirmed the identity of the BPBD-associated bacterium. Pathogenicity assays verified the ability of these isolates to produce discoloration, spotting, and empty grains, symptoms associated with BPBD. Antibiotic assays showed that EC-EELS-01 isolate was sensitive to ciprofloxacin and tetracycline, and resistant to polymyxin. The dissemination and prevalence of B. glumae were confirmed in the rice-producing areas of El Oro, Cañar, Guayas, and Los Rios provinces. This research will serve to develop genetic studies to characterize the population in the B. glumae isolates in Ecuador.

Highlights

Bacterial panicle blight disease (BPBD), called bacterial grain rot or panicle blight, is considered a major disease of rice (Oryza sativa L.), causing severe damage to this crop worldwide (Nandakumar et al, 2009; Lee et al, 2016a; Bigirimana et al, 2015; Mondal et al, 2015)
Bacterial panicle blight disease was first reported in Japan, causing grain rotting and seedling blight on rice (Goto and Ohata, 1956)
All isolates grown on nutrient-broth yeast medium (NBY) medium at 40° C and on yeast dextrose calcium carbonate (YDC) medium were light cream in color (Table 2)

Summary

Introduction

Bacterial panicle blight disease (BPBD), called bacterial grain rot or panicle blight, is considered a major disease of rice (Oryza sativa L.), causing severe damage to this crop worldwide (Nandakumar et al, 2009; Lee et al, 2016a; Bigirimana et al, 2015; Mondal et al, 2015). Rice is considered its main host plant (Lee et al, 2016a; Magbanua et al, 2014; Sharma et al, 2013) It can survive on rice leaves and sheaths, spreading upwards as the plant grows (Ham et al, 2011).

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