Prevalence of autotransporters in Escherichia coli: what is the impact of phylogeny and pathotype?

Abstract

Autotransporter (AT) proteins are widespread surface-exposed or secreted factors in Escherichia coli. Several ATs have been correlated with pathogenesis or specific phylogenetic lineages. Therefore, an application as biomarkers for individual extraintestinal pathogenic E.coli (ExPEC) or intestinal pathogenic E.coli (IPEC) has been proposed. To put this assumption on a solid foundation, we analyzed 111 publicly available E. coli genome sequences and screened them bioinformatically for the presence of 18 ATs. We determined the highest AT prevalence per strain in phylogroup B2 isolates and showed that AT distribution correlates rather with phylogenetic lineages than with pathotypes. Although a strict dependence between AT prevalence and pathotype was not observed, EspP, EhaA, and EhaG cluster with IPEC of phylogroup B1 and E, respectively, whereas UpaH is predominantly present in ExPEC of phylogroup B2. Furthermore, PicU, SepA, UpaB, UpaI, and UpaJ were associated with phylogroup B2. We detected UpaI and its positional ortholog EhaC in 93% of the E.coli strains tested. This AT variant is thus the most prevalent in E.coli irrespective of pathotype or phylogenetic background. Compared with the ATs UpaB, UpaC, and UpaJ of uropathogenic E.coli strain 536, UpaI had redundant functions, contributing to autoaggregation, biofilm formation, and binding to extracellular matrix proteins. The functional redundancy and wide distribution of ATs among pathogenic and non-pathogenic E.coli indicates that ATs cannot generally be regarded as specific biomarkers and virulence factors. Our results demonstrate that phylogeny has a bigger impact on the distribution of AT variants in E.coli than initially thought, especially in ExPEC.