PREVALENCE, ANTIMICROBIAL SUSCEPTIBILITY PATTERN AND RISK FACTORS OF MRSA ISOLATED FROM CLINICAL SPECIMENS AMONG MILITARY PATIENTS AT 48 MEDICAL COMPOUND IN SANA'A CITY-YEMEN

Ahmed Mohammed Ali Al-Safani

doi:10.22270/ujpr.v3i3.165

Abstract

Objective: Methicillin-resistant strains of S. aureus evolved in the 1970 and have troubled hospitals worldwide with persistent infections in patients. The objectives of this study were to determine the prevalence, antimicrobial susceptibility pattern and risk factors of MRSA isolated from clinical specimens among military patients at 48 Medical Compound in Sana'a city - Yemen. Methods: The study included 233 patients of whom suffering from Staphylococcus aureus infections. Specimens and data collected from November 2016 to November 2017. Standard methods of isolation and identifications were used to isolate bacteria in pours culture then Staphylococcus aureus were identifying using standard cultural techniques. MRSA was determined by the disc diffusion method to oxacillin and antimicrobial susceptibility testing was performed by the disc diffusion method for selected antibiotics. Results: The prevalence rate of MRSA was 19.3% and there was significant association between MRSA and older age patients, and surgical site infections. There was higher rate of antibiotics resistant for tested antibiotics in MRSA isolates comparing with lower rate of antibiotics resistant in MSSA. 60% of the MRSA isolates were resistant to vancomycin. They were also susceptible to erythromycin and rifampicin (100%), but showed resistance to Cotrimoxazole and Gentamycine. Conclusion: In conclusion, the emergence of S. aureus isolates resistant to vancomycin and other wide range of antibiotics have raised MRSA in Yemen into a multi-drug-resistant ‘Superbug”, making it more and more dangerous than ever in hospital environments. Regular surveillance of hospital associated infections and monitoring antibiotic sensitivity pattern and strict drug policy for antibiotics used within and outside the hospital environments are recommend. Peer Review History: Received 20 June 2018; Revised 29 June; Accepted 5 July, Available online 15 July 2018 UJPR follows the most transparent and toughest ‘Advanced OPEN peer review’ system. The identity of the authors and, reviewers will be known to each other. This transparent process will help to eradicate any possible malicious/purposeful interference by any person (publishing staff, reviewer, editor, author, etc) during peer review. As a result of this unique system, all reviewers will get their due recognition and respect, once their names are published in the papers. We expect that, by publishing peer review reports with published papers, will be helpful to many authors for drafting their article according to the specifications. Auhors will remove any error of their article and they will improve their article(s) according to the previous reports displayed with published article(s). The main purpose of it is ‘to improve the quality of a candidate manuscript’. Our reviewers check the ‘strength and weakness of a manuscript honestly’. There will increase in the perfection, and transparency. Received file: Reviewer's Comments: Average Peer review marks at initial stage: 6.5/10 Average Peer review marks at publication stage: 8.5/10 Reviewer(s) detail: Dr. Tanveer Ahmed Khan, Hajvery University, Lahore, Pakistan, tanveerahmedkhan754@gmail.com Dr. Wilman Ismael Carrillo Teran, Technical University of Ambato Avda, Ecuador, wilmanct@gmail.com Similar Articles: BIOFILM FORMATION AND ANTIBIOTIC SUSCEPTIBILITY OF UROPATHOGENS IN PATIENTS WITH CATHETER ASSOCIATED URINARY TRACT INFECTIONS IN IBB CITY -YEMEN

Highlights

INTRODUCTIONStaphylococcus aureus (S. aureus) is a bacterium of significant importance because of its ability to cause a wide range of diseases and capacity to adapt to diverse environmental forms[1]
Total S. aureus identification of isolates was made according to standard methods[9] for any potential clinically significant growth appear on the culture media on the Survey procedure and Laboratory Analysis This study was conducted during a period of one year, starting in November 2016 and ending in November 2017 at 48 Medical compounds in Sana'a city-Yemen
Table 1, Table 2, and Table 3 show the prevalence of Methicillin-susceptible S. aureus (MSSA) and MRSA among isolated coagulase positive (S. aureus), the potential associated factors of MRSA in patients with S. aureus infection and the association between prevalence of MRSA and type of hospital wards

Summary

INTRODUCTION

Staphylococcus aureus (S. aureus) is a bacterium of significant importance because of its ability to cause a wide range of diseases and capacity to adapt to diverse environmental forms[1]. MRSA carries a uniquely effective antibiotic resistance mechanism that can protect the microorganisms against all members of β-lactam antibiotics This makes infections caused by these pathogens very difficult to manage and costly to treat[5,6]. The objectives of this study were to determine the prevalence, antimicrobial susceptibility pattern and risk factors of MRSA isolated from clinical specimens among military patients at 48 Medical Compound in Sana'a city-Yemen. Total S. aureus identification of isolates was made according to standard methods[9] for any potential clinically significant growth appear on the culture media on the Survey procedure and Laboratory Analysis This study was conducted during a period of one year, starting in November 2016 and ending in November 2017 at 48 Medical compounds in Sana'a city-Yemen. Odd's ratio (OR), confidence interval (CI) were added to estimate the risk factors of contracting MRSA

RESULTS

DISCUSSION

CONCLUSION