Prevalence and subtypes of Influenza A Viruses in Wild Waterfowl in Norway 2006-2007

Anna Germundsson,Christine Monceyron Jonassen,Monika Jankowska Hjortaas,Knut I Madslien,Kjell Handeland

doi:10.1186/1751-0147-52-28

Anna Germundsson, Christine Monceyron Jonassen + Show 3 more

Open Access

https://doi.org/10.1186/1751-0147-52-28

Copy DOI

Abstract

The prevalence of influenza A virus infection, and the distribution of different subtypes of the virus, were studied in 1529 ducks and 1213 gulls shot during ordinary hunting from August to December in two consecutive years, 2006 and 2007, in Norway. The study was based on molecular screening of cloacal and tracheal swabs, using a pan-influenza A RT-PCR. Samples found to be positive for influenza A virus were screened for the H5 subtype, using a H5 specific RT-PCR, and, if negative, further subtyped by a RT-PCR for the 3'-part of the hemagglutinin (HA) gene, encompassing almost the entire HA2, and the full-length of the neuraminidase (NA) gene, followed by sequencing and characterization. The highest prevalence (12.8%) of infection was found in dabbling ducks (Eurasian Wigeon, Common Teal and Mallard). Diving ducks (Common Goldeneye, Common Merganser, Red-breasted Merganser, Common Scoter, Common Eider and Tufted Duck) showed a lower prevalence (4.1%). In gulls (Common Gull, Herring Gull, Black-headed Gull, Lesser Black-headed Gull, Great Black-backed Gull and Kittiwake) the prevalence of influenza A virus was 6.1%. The infection prevalence peaked during October for ducks, and October/November for gulls. From the 16 hemagglutinin subtypes known to infect wild birds, 13 were detected in this study. Low pathogenic H5 was found in 17 dabbling ducks and one gull.

Highlights

The prevalence of influenza A virus infection, and the distribution of different subtypes of the virus, were studied in 1529 ducks and 1213 gulls shot during ordinary hunting from August to December in two consecutive years, 2006 and 2007, in Norway
High prevalence of infection was found in dabbling ducks (189/ 1480, 12.8%), whereas lower prevalence were seen in diving ducks (2/49, 4.1%) and gulls (74/1213, 6.1%)
The subtype H5 was found in 22 birds, and further sequencing of the cleavage site of the HA gene identified all of them as low-pathogenic strains (LPAI). Seventeen of these samples were detected in Mallards, one in Eurasian Wigeons, three in Common Teals, and one in Herring Gulls

Summary

Introduction

The prevalence of influenza A virus infection, and the distribution of different subtypes of the virus, were studied in 1529 ducks and 1213 gulls shot during ordinary hunting from August to December in two consecutive years, 2006 and 2007, in Norway. In Sweden the prevalence of influenza A viruses in Mallards were 3-fold higher as compared to the Netherlands during the same time of the year [3]. Samples with a ct-value of 38 or below were considered to be positive for influenza A virus.

Results

Conclusion