Abstract
Objective: The objectives of this study was to investigate phenotypically the prevalence of Staphylococcus aureus and Methicillin Resistance S. aureus (MRSA) in ruminants and animal handlers in Maiduguri, Nigeria. Materials and methods: A total of 937 samples (900 nasal and skin swabs of cattle and 37 humans samples) were collected in Maiduguri metropolis. The samples were inoculated onto mannitol salt agar (MSA) and blood agar, and the cultrue plates were incubated at 37 o C for 24 h. The S. aureus colonies showing golden yellow color on MSA were primarily identified as S. aureus , which were then subjected for catalase and coagulase tests. All S. aureus were finally screened for the presence of MRSA on oxalate resistant screening agar (ORSAB) medium. Antimicrobial susceptibility of the MRSA were measured by disc diffusion method. Results: The overall prevalence of S. aureus was 44.3% (n=414/937). The MRSA could be detected in 12.1% (n=113/937) samples. Considerign different species, S. aureus could be isolated from 137 (45.6%), 148 (49.3%) and 119 (39.7%), 11 (29.7%) cattle, sheep, goat and humans, respectively. In ruminants, 27.7% (n-83/300) bulls were positive for S. aureus . Similarly, 10.7% (n=32/300) and 22.3% (n=67/300) were positive for S. aureus in rams and bucks, respectively. On the other hand, 18% (n=54/300), 38.7% (n=116/300) and 17.3% (n=52/300) samples from cows, ewes and does were positive for S. aureus . The highest MRSA could be isolated from ewes (9.3%; n=28/300) follwed by bulls (7%; n=21/300) and bucks (6.7%; n=20/300). In humans, 13.5% (n=5/37) samples were positive for MRSA. Conclusion: S. aureus and MRSA infections in ruminants and animal handlers are documented in this study. The MRSA may exert public health threat to humans. http://doi.org/10.5455/javar.2016.c145
Highlights
Staphylococcus aureus (S. aureus) is a microbe of potential public health dread because of its inherent ability to infect and cause life daunting infections and to survive in diverse milieu (Waidvogel, 2000; Pantosti, 2012)
137 (45.7%) cattle, 148 (49.3%) sheep, 119 (39.7%) goats, and 11 (29.7%) humans were positive for S. aureus, respectively
45.4% isolates were positive for catalase, 44.3% samples were positive for coagulase, and 12.1% showed intense blue coloration on Oxacillin resistant screening agar base (ORSAB) media growth
Summary
Staphylococcus aureus (S. aureus) is a microbe of potential public health dread because of its inherent ability to infect and cause life daunting infections and to survive in diverse milieu (Waidvogel, 2000; Pantosti, 2012). The well-being of consuming clean meat from ruminants can be influenced reliably by unsanitary nature of ruminant handlers and contact with unclean surfaces such as worktables and grubby knives (Albuquerque et al, 2007). Enterotoxin secreted by this microbe is accountable for some food poisoning (Griffeth and Morris, 2008) with detrimental public health risk, and causing substantial morbidity and mortality (Waidvogel, 2000; Pantosti, 2012)
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Journal of Advanced Veterinary and Animal Research
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
