Abstract

Infections by methicillin-resistant Staphylococcus aureus (MRSA) are continuously expanding within the community. Chicken meat is usually contaminated by MRSA, and this contaminated chicken meat is an important source of foodborne infections in humans. In this study, a cross-sectional supershop survey was conducted to determine the prevalence and antimicrobial resistance pattern of MRSA in 113 domestic frozen chicken meat samples purchased from nine branded supershops available in five divisional megacities of Bangladesh. The study also focused on the determination of methicillin resistance gene in MRSA isolates. S. aureus was identified by standard culture-based and molecular methods, and subjected to antimicrobial susceptibility testing. MRSA was screened by cefoxitin disk diffusion test. Methicillin resistance gene was identified by PCR. Of samples, 54.9% were positive for S. aureus, and, of these, 37.1% isolates were identified as MRSA. All the isolates were multidrug resistant (MDR): 52.2% were resistant to 6–8 antimicrobial classes, and 47.8% isolates to 9–12 classes. Three (3.2%) isolates of S. aureus were possible extensively drug resistant. The highest rates of resistance were observed against cefoxitin (100%), followed by nalidixic acid, ampicillin and oxacillin (97.7%), colistin (91.3%), amoxicillin-clavulanic acid and amoxicillin (87%), penicillin-G and cloxacillin (82.6%), oxytetracycline (78.3%), and cefixime (73.9%). Screening of methicillin resistance gene revealed that 43.5% isolates of MRSA were positive for mecA gene. The high prevalence of MDR MRSA in frozen chicken meat samples in this study emphasizes the need for better sanitary education of food handlers in hygienic practices focusing on their potential role as reservoirs and spreaders of MRSA.

Highlights

  • Foodborne diseases (FBDs) are a significant general well-being concern worldwide [1].The World Health Organization (WHO) characterizes FBD as “disease of infectious or toxic nature caused by, or thought to be caused by, the consumption of food or water” [1]

  • The present study demonstrated that the occurrence of methicillin-resistant Staphylococcus aureus (MRSA) as well as methicillin-susceptible S. aureus (MSSA) varied among different brands of frozen chicken meat

  • This study described the association between AMR phenotypes and presence of methicillin resistance gene (mecA) gene in MRSA and methicillin-sensitive Staphylococcus aureus (MSSA) isolated from frozen chicken meat samples

Read more

Summary

Introduction

Foodborne diseases (FBDs) are a significant general well-being concern worldwide [1]. The World Health Organization (WHO) characterizes FBD as “disease of infectious or toxic nature caused by, or thought to be caused by, the consumption of food or water” [1]. S. aureus is an opportunistic pathogen in human and animals, and is the third largest cause of food related illness throughout. Microorganisms 2021, 9, 636 the world [3,4]. S. aureus can cause a wide spectrum of infections, from superficial skin infections to severe, and probably fatal, invasive illness [5]. Ingestion of staphylococcal enterotoxins created in food by S. aureus enterotoxigenic strains results in staphylococcal food poisoning that can be thought of as perhaps the most widely recognized foodborne infection [3]. Among diverse kinds of foodstuffs, chicken meat handling with poor hygienic practice is exceptionally related with contamination of S. aureus enterotoxin [3]

Objectives
Methods
Results
Discussion
Conclusion
Full Text
Published version (Free)

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call