Abstract
Prevalence and molecular identification of Cryptosporidium spp in cattle in Baghdad province, Iraq
Highlights
Cryptosporidium spp. are obligate intracellular protozoan parasites that can infect a wide range of animals and human hosts worldwide [1]
Nested PCR Genomic DNA samples obtained from cattle fecal samples were subjected to molecular analysis by nested PCR using 18S rRNA gene specific primers in order to identify the species of Cryptosporidium
Nested PCR of all 100 samples employed in the study exhibited distinct band of 318 bp on agarose gel confirming the presence of Cryptosporidium spp (Figure 1)
Summary
Cryptosporidium spp. are obligate intracellular (extracytoplasmic) protozoan parasites that can infect a wide range of animals and human hosts worldwide [1]. The molecular tools can identify more precise definitions of host-adapted specificity, transmission pathways, and zoonotic possibility of Cryptosporidium spp. Cattle are commonly infected with 4 Cryptosporidium spp. which include C. parvum, C. andersoni C. bovis and C. ryanae [1,7]. Given this diversity of Cryptosporidium species in cattle and differing zoonotic potential, it is important to be able to estimate their prevalence and distribution in particular geographic regions using molecular tools [8]
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