Prevalence and Molecular detection of some virulence genes among Salmonella species Isolated from Poultry

Abstract

The current study aimed to isolate the salmonella species from different organs of broilers with complete phenotypic identification of the recovered isolates. After that all recovered isolates were tested for detection of some genes responsible for pathogencity.A total of 250 samples from broilers farms were examined bacteriologically for isolation of salmonella. All recovered isolates were identified phenotypically, biochemically and serologically. PCR technique was used for detection of invA, hilAand avrA genes. Bacteriological examination of samples revealed that Salmonella was recovered in 34 samples out of 250 samples (13.6%). Twelve S. Typhimurium, 8 S. Kentucky, 5 S. Newport, 2 S. Tamale, 2 S. Enteritidis, 1 S. Molade,2 S. Takoradi, 1 S. Virchow and 1 S. Inganda were isolated from broilers with percentage of (35.29%), (23.5%), (14.7%), (5.88%), (5.88%), (2.94%), (5.88%), (2.94%) and (2.94%) respectively. All tested Salmonella serovars showed positive amplification at 284 bp, 150 bp and 422 bp fragments which specific for the invAgene (common gene), hilAgene and avrA gene respectively, with total percentage (100%) from examined samples.