Abstract
Shiga toxin-producing Escherichia coli (STEC) are zoonotically important pathogen which causes hemorrhagic colitis, diarrhea, and hemolytic uremic syndrome in animals and humans. The present study was designed to isolate and identify the STEC from fecal samples of diarrheic cattle. A total of 35 diarrheic fecal samples were collected from Bangladesh Agricultural University (BAU) Veterinary Teaching Hospital. The samples were primarily examined for the detection of E. coli by cultural, morphological and biochemical characteristics, followed by confirmation of the isolates by Polymerase Chain Reaction (PCR) using gene specific primers. Later, the STEC were identified among the isolated E. coli through detection of Stx-1 and Stx-2 genes using duplex PCR. Out of 35 samples, 25 (71.43%) isolates were confirmed to be associated with E. coli, of which only 7 (28%) isolates were shiga toxin producers, and all of them were positive for Stx-1. However, no Stx-2 positive isolate could be detected. From this study, it may be concluded that cattle can act as a reservoir of STEC which may transmit to human or other animals.J. Bangladesh Agril. Univ. 14(1): 63-68, June 2016
Highlights
Escherichia coli (E. coli) is a Gram-negative, rod-shaped, flagellated, motile, oxidase negative, facultative anerobic organism, and is classified under the family Enterobacterioceae (Riley et al, 1983)
Pathogenic E. coli are grouped into enterotoxic E. coli (ETEC), enteropathogenic E. coli (EPEC), enteroinvasive E. coli (EIEC), enteroaggregative E. coli (EAggEC ) and enterohaemorrhagic E. coli (EHEC) (Riley et al, 1983)
Often they are referred to depending on their toxin producing capabilities, known as verocytotoxin producing E. coli (VTEC) or shiga-like toxin producing E. coli (STEC)
Summary
Escherichia coli (E. coli) is a Gram-negative, rod-shaped, flagellated, motile, oxidase negative, facultative anerobic organism, and is classified under the family Enterobacterioceae (Riley et al, 1983). No report has been observed on the detection or isolation of STEC from diarrheic cattle in Bangladesh. Considering the above points, the present study was conducted to isolate and detect shiga toxin producing E. coli from diarrheic cattle. Colony characteristics of E. coli observed on EMB and MC agars were similar to the findings of other authors (Rehman et al, 2014; Hassan et al, 2014; Mamun et al, 2016; Tanzin et al, 2016; Elafify et al, 2016; Islam et al, 2016).
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
