Abstract
Matter: This is the first study in Al-Anbar governorate with this design aim to the detection of rotavirusgroup A (ROVA).Method: A total of (150) stool samples obtained from children <5 years with acute gastroenteritis wererandomly collected from Maternity and Children at al-Anbar governorate from (1- 9-2019 to 1-2-2020);Rotavirus was detected by rapid test for stool samples and real-time polymerase chain reaction for bloodand serum.Results: Out of total 150 stool sample there were 101(67.3%), 49(32.6%) Negative and positive resultrespectively, from rapid test. The result of RT-PCR from serum & blood were Thirty-one (31)81.57% of(38) samples positive for rotavirus & eight samples were no ct there was Significant value (0.0016)(0.0075)between PCR (blood, serum) test and Rapid test under p<0.05. Based on that RT-PCR was more sensitive& specific than rapid test
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