Abstract

Due to its ability to spread disease from one host to another, tick infestation is the most frequent issue pet owners’ deal with and should be taken seriously. The goal of the current study was to ascertain the prevalence of tick infestation in dogs in Agartala as well as its molecular identity and characterisation. A total of 1776 dogs of various breeds, ages, sexes, and categories were included in the study, which was conducted from March 2022 to February 2023 over the course of a calendar year. The total prevalence of ticks was reported to be 62.12%. According to study on breed, sex, age, and category, German spitz, male dogs, dogs under 1 year of age and stray dogs had the highest frequency of tick infestation. All throughout the year, tick infestations were seen, with the monsoon season seeing the highest frequency. Based on 16S rRNA gene amplification and sequence analysis, molecular identification revealed that all isolated ticks were members of the Rhipicephalus sanguineus species. Using a subset of the 16S rRNA gene, phylogenetic analysis revealed that R. sanguineus (OR244468.1) from Agartala (TrRS-PB) was part of the same clade as isolates from Kerala and Assam.

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