Prevalence and molecular characterisation of Echinococcus granulosus in disposed of bovine carcasses in Punjab, India.

Abstract

Cystic echinococcosis is an important parasitic zoonosis across the globe. The disease is caused by Echinococcus granulosus sensu lato. Free access of dogs to the disposed of bovine carcasses is an important conducive factor for the persistence of E. granulosus in the environment, and thus, stray dogs play a critical role in the disease transmission in developing countries including India. There is lack of information on the status of E. granulosus in the disposed of bovine carcasses in India; however, this information would be helpful to plan future strategies and policies to control this parasite. We examined 85 bovine carcasses from the 7 selected carcasses disposal sites in Punjab. During the postmortem, internal organs were examined for the presence of hydatid cysts. Molecular characterization of Echinococcus spp. was performed by PCR followed by sequencing of partial COI gene. We report 21% (18/85) prevalence of hydatidosis in dispose of bovine carcasses. Age was reported as the significant risk factor (Chi square = 22.5, df = 2, p = 0) for hydatidosis in the studied population and this might be due to higher cumulative exposure to the environmental parasitic stage (E. granulosus eggs) with increase in age. Phylogenetic analysis showed that G1 (predominantly) and G3 strains of E. granulosus are circulating in bovine population in Punjab. Further, studies are needed to determine the strains of E. granulosus circulating in the stray dogs. Measures and policies to combat this problem in India should involve one health approach: a collaborative effort of medical and veterinary physicians.