Year-end Sale % OFF 
Abstract
Here, we aim to determine the prevalence of Salmonella contamination of poultry meat from butcheries of the province of Skikda and to investigate antibiotic resistance. Salmonella spp. isolates were screened from 70 samples, including chicken breasts (n = 40 samples) and chicken thighs (n = 30 samples) collected from 14 butcheries. All suspected Salmonella colonies from selective media were confirmed by MALDI-TOF MS and serotyped. The susceptibility profile to 16 antibiotics was studied. According to the antibiotic susceptibility results, resistance genes were investigated by standard PCR targeting various genes such as blaSHV, blaTEM, aac3, aac6-Ibcr, aad, qnrA and qnrB. Of the 14 butcheries studied, samples from eight butcheries were contaminated with Salmonella (57.14%). 19 Salmonella strains were isolated, including five serotypes with a predominance of Kentucky serotype (n = 9), Enteridis (n = 3), followed by Heidelberg (n = 3), Virchow (n = 3), and Manhattan (n= 1). All isolates were resistant to Rifampicin (100%; n = 19), and to other antibiotics such as Ciprofloxacin (47.36%), Amoxicillin-clavulanic acid (47.36%; n = 9), Amoxicillin, (47.36%; n = 9), Ticarcillin-clavulanic acid (47.36%; n = 9), and Gentamycin (47.36%; n = 9). All isolates showing multidrug resistance (47.36%; n = 9) were positive by PCR to the blaTEM-1 β-lactamase gene, from which 8 strains carried the aminoglycoside resistance aad7 gene. However, none was positive for the tested blaSHV, Aac3, Aac6-Ibcr, qnrA, qnrB, ArmA and ArmB genes. Our findings show a worrying rate of Salmonella contamination of poultry meats.
Highlights
Salmonella is one of the most important causes of foodborne diseases worldwide
Of the 14 butcher shops studied, eight had poultry meat contaminated with Salmonella, resulting in a prevalence rate of 57.14%
Nineteen Salmonella strains were isolated from poultry meat samples and confirmed by MALDI-TOF MS and gave very good scores ranging from 2.00 to 3.00
Summary
Salmonella is one of the most important causes of foodborne diseases worldwide. It is frequently associated with consumption of contaminated products such as poultry, eggs, meat, milk and seafood [1]. In Algeria, the poultry meat contamination occurs during the transport of live birds, their housing, slaughter and marketing without compliance with basic hygiene criteria. This meat is generally implicated in human salmonellosis outbreaks causing acute gastroenteritis, especially in young and immunodeficient patients [6]. The latter carry multiple antibiotic resistance genes that are transferable to other Salmonella strains and other bacterial species [7] In this scope, the present study was undertaken to study the prevalence of Salmonella contamination in marketed poultry meat in Skikda province and to
Sign-in/Register to view highlights & summary 
Published Version (
Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
