Prevalence and Distribution of Dengue Virus in Aedes aegypti in Yogyakarta City before Deployment of Wolbachia Infected Aedes aegypti.

Ayu Rahayu,Utari Saraswati,Edwin Widyanto Daniwijaya,Adi Utarini,Dwi Satria Wardana,Eggi Arguni,Citra Indriani,Dian Aruni Kumalawati,Iva Fitriana,Riris Andono Ahmad,Warsito Tantowijoyo,Endah Supriyati,Anwar Rovik,Sigit Setyawan,Rio Hermantara

doi:10.3390/ijerph16101742

Ayu Rahayu, Utari Saraswati + Show 13 more

Open Access

https://doi.org/10.3390/ijerph16101742

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Abstract

Indonesia is one of the countries where dengue infection is prevalent. In this study we measure the prevalence and distribution of dengue virus (DENV) DENV-infected Aedes aegypti in Yogyakarta City, Indonesia, during the wet season when high dengue transmission period occurred, as baseline data before implementation of a Wolbachia-infected Aedes aegypti trial for dengue control. We applied One-Step Multiplex Real Time PCR (RT-PCR) for the type-specific-detection of dengue viruses in field-caught adult Aedes aegypti mosquitoes. In a prospective field study conducted from December 2015 to May 2016, adult female Aedes aegypti were caught from selected areas in Yogyakarta City, and then screened by using RT-PCR. During the survey period, 36 (0.12%) mosquitoes from amongst 29,252 female mosquitoes were positive for a DENV type. In total, 22.20% of dengue-positive mosquitoes were DENV-1, 25% were DENV-2, 17% were DENV-3, but none were positive for DENV-4. This study has provided dengue virus infection prevalence in field-caught Aedes aegypti and its circulating serotype in Yogyakarta City before deployment of Wolbachia-infected Aedes aegypti.

Highlights

Dengue is the fastest spreading mosquito borne disease in the world and is endemic in most tropical countries with an estimated 96 million symptomatic cases annually [1]
We showed that infection status of dengue virus in Aedes aegypti population is not correlated with mosquito density
Our results showed the preliminary data of dengue virus (DENV) circulating in the Aedes aegypti mosquito population in Yogyakarta City

Summary

Introduction

Dengue is the fastest spreading mosquito borne disease in the world and is endemic in most tropical countries with an estimated 96 million symptomatic cases annually [1]. Health Organization (WHO) (2009) an estimated 2.5 billion people live in areas where there is a risk of dengue transmission, with about 975 million of those living in built-up areas in different countries. Res. Public Health 2019, 16, 1742; doi:10.3390/ijerph16101742 www.mdpi.com/journal/ijerph

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