Prevalence and Characterization ofStreptococcus pyogenesClinical Isolates from Different Hospitals and Clinics in Mansoura

Abstract

Streptococcus pyogenesare associated with many bacterial diseases in both humans and animals and are capable of causing a multitude of human diseases.S. pyogenesisolates were identified by their bacitracin sensitivity, positivespy1258detection, and positive GAS latex agglutination. Different isolates were typed serotypically and genotypically by BOX-PCR. Different virulence factors were identified inS. pyogenesisolates. In addition, antimicrobial resistance was tested to eleven different antibiotics. Furthermore, the resistance mechanisms were determined phenotypically by the disc diffusion method. Finally, the correlation between both molecular and serotypes identified and the profile of virulence factors and clinical and geographical sources was determined for all isolates. Thirty-eightS. pyogenesisolates were collected from different clinical sources. Resistance testing indicated high resistance to mostly used antibiotics except amoxicillin/clavulanic acid, amoxicillin, and ampicillin. Serotyping results indicated five different serotypes, M1, M2, M3, M4, and M6, inS. pyogenesisolates, while six isolates were identified as untypeable. In addition, positive PCR results identified most of the tested SAgs genes in whichspeJgene was mostly identified followed byspeI, speC,andssagenes being identified in 81.6%, 63.3%, 60.5%, and 60.5%, respectively. However,speHwas the least detected. In contrast,speL, speM, andsmeZgenes could not be detected in all tested isolates. Finally, BOX-PCR molecular typing was a more effective clustering method when compared to the serotyping method in allS. pyogenes.In conclusion, the isolates in this study were highly resistant to mostly used antibiotics. M1 was the most identified serotype. No significant association was found between serotypes, BOX-PCR cluster groups, and SAgs genes profiles. However, by the application of BOX-PCR, effective molecular typing was obtained.