Prevalence and characterization of Listeria monocytogenes isolated from retail-level ready-to-eat foods in South China

Abstract

Listeria monocytogenes is an important food-borne pathogen causing meningitis, meningoencephalitis and abortion. To assess the potential risk to consumer health, the presence of L. monocytogenes was investigated using qualitative and quantitative methods. Ten (6.33%) of 158 retail RTE food samples were positive for L. monocytogenes and the contamination levels were less than 10 MPN/g,while none of 65 dairy products was positive for L. monocytogenes. The 37 strains were grouped into five clusters and two singletons, five clusters and two singletons, and three clusters and one singleton by enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR) and RAPD fingerprint respectively, at similarity coefficient of 80%. The susceptibility test showed that 83.8% were susceptible to 15 antimicrobials; two were penicillin-resistant, and one was multidrug-resistant to kanamycin, tetracycline, sulfamethoxazole, rifampin, gentamycin, penicillin, and ampicillin. Virulent L. monocytogenes that possess partial antimicrobial resistance, and serotypes frequently associated with listeriosis were recovered from RTE foods. Consumers may, therefore, be exposed to potential risks of L. monocytogenes infection in South China. This study contributed to the prevalence and contamination levels of L. monocytogenes in RTE foods in South China for the first time, providing baseline information for Chinese regulatory authorities to formulate a regulatory framework for controlling L. monocytogenes to improve the microbiological safety of RTE foods.