Prevalence and Characteristics of Phenicol-Oxazolidinone Resistance Genes in Enterococcus Faecalis and Enterococcus Faecium Isolated from Food-Producing Animals and Meat in Korea.

Eiseul Kim,Hyo-Sun Kwak,So-Won Shin,Gun-Jo Woo,Min-Hyeok Cha,Yoon-Soo Gwak,Hae-Yeong Kim,Seung-Min Yang

doi:10.3390/ijms222111335

Eiseul Kim, Hyo-Sun Kwak + Show 6 more

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https://doi.org/10.3390/ijms222111335

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Abstract

The use of phenicol antibiotics in animals has increased. In recent years, it has been reported that the transferable gene mediates phenicol-oxazolidinone resistance. This study analyzed the prevalence and characteristics of phenicol-oxazolidinone resistance genes in Enterococcus faecalis and Enterococcus faecium isolated from food-producing animals and meat in Korea in 2018. Furthermore, for the first time, we reported the genome sequence of E. faecalis strain, which possesses the phenicol-oxazolidinone resistance gene on both the chromosome and plasmid. Among the 327 isolates, optrA, poxtA, and fexA genes were found in 15 (4.6%), 8 (2.5%), and 17 isolates (5.2%), respectively. Twenty E. faecalis strains carrying resistance genes belonged to eight sequence types (STs), and transferability was found in 17 isolates. The genome sequences revealed that resistant genes were present in the chromosome or plasmid, or both. In strains EFS17 and EFS108, optrA was located downstream of the ermA and ant(9)-1 genes. The strains EFS36 and EFS108 harboring poxtA-encoding plasmid cocarried fexA and cfr(D). These islands also contained IS1216E or the transposon Tn554, enabling the horizontal transfer of the phenicol-oxazolidinone resistance with other antimicrobial-resistant genes. Our results suggest that it is necessary to promote the prudent use of antibiotics through continuous monitoring and reevaluation.

Highlights

Enterococci are symbiotic bacteria of the gastrointestinal tract of humans and animals [1]
Enterococci harboring the phenicol-oxazolidinone resistant genes optrA, poxtA, cfr, and fexA were detected from 282 E. faecalis and 45 E. faecium isolates
OptrA, poxtA, and fexA were detected in 15 isolates (4.6%), 8 isolates (2.5%), and 17 isolates (5.2%), respectively, confirming that these genes were distributed at a low level

Summary

Introduction

Enterococci are symbiotic bacteria of the gastrointestinal tract of humans and animals [1]. The use of florfenicol, phenicol antibiotics, has been increasing in animals to treat diseases caused by E. faecalis and E. faecium infections [5]. The second mechanism involves the acquisition of transferable resistance genes, such as optrA and poxtA, encoding the adenosine triphosphate-binding cassette F, as well as cfr, which encodes a methyltransferase [8]. These genes are considered as multiple resistance genes; optrA confers resistance to phenicols (chloramphenicol and florfenicol) and oxazolidinones (linezolid and tedizolid), poxtA confers resistance to phenicol-oxazolidinone-tetracycline, and cfr confers resistance to phenicols, oxazolidinones, lincosamides, streptogramin A, and pleuromutilins [8]. These genes are considered as multiple resistance genes; optrA confers resistance to phenicols (chloramphenicol and florfenicol) and oxazolidinones (linezolid and tedizolid), poxtA confers resistance to phenicol-oxazolidinone-tetracycline, and cfr confers resistance to phenicols, oxazolidinones, lincosamides, streptogramin A, and pleuromutilins [8]. optrA, poxtA, and cfr genes present as part of a plasmid or as a transposon composite; it is known that the possibility of transferring these genes to other bacteria is very high [5]

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