Abstract
The precursors, 5-Aminolevulinic Acid (ALA) and Porphobilinogen (PBG), are involved in the heme biosynthetic pathway...
Highlights
The precursors, 5-Aminolevulinic Acid (ALA) and Porphobilinogen (PBG), are involved in the heme biosynthetic pathway
The ALA and PBG signals were significantly increased after elution of urine through silica Solid Phase Extraction (SPE) cartridges
Silica SPE cartridges were effective at removing ion suppression, improving the sensitivity and reliability of the method
Summary
The precursors, 5-Aminolevulinic Acid (ALA) and Porphobilinogen (PBG), are involved in the heme biosynthetic pathway. ALA and PBG have previously been measured by colorimetric methods using ion chromatography and complexation with Ehrlich’s reagent [7,8], by High Performance Liquid Chromatography (HPLC) [9], and more recently by liquid chromatography mass spectrometry (LC-MS/MS) [1,2,10]. We previously measured ALA and PBG colorimetrically using a kitset method. The precursors for heme, 5-aminolevulinic acid (ALA) and porphobilinogen (PBG), are elevated in the urine of patients during an attack of one of the acute porphyrias. ALA and PBG can be measured directly by liquid chromatography tandem mass spectrometry (LC-MS/MS) without derivatization. The aim was to develop a robust LC-MS/MS method for measuring ALA and PBG without the need for derivatization
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
