Prevalence and antimicrobial susceptibility of Pasteurella multocida isolated from goats in Can Tho city

Abstract

A total of 289 goats’ nasal swabs were collected from March to May 2023 to determine the prevalence of Pasteurella multocida in goats raised on medium-scale farms in Can Tho city. There were 143 samples representing 49.48% positive for Pasteurella multocida. Of 143 positive samples, 64 strains were selected to examine the antimicrobial susceptibility using the disk diffusion method. The results showed that those strains were still sensitive to 6/7 examined antibiotics, especially doxycycline (100%); however, they were highly resistant to ampicillin (53.13%). There were 9 phenotypes of antibiotic resistance (60.94%), with the most common patterns of ampicillin and ampicillin + amoxicillin/clavulanic acid (23.44%).By using PCR assay to detect antibiotic resistance genes, the results revealed that sulII gene was the most frequent detection (67.19%). A total of 11 resistance genotypes detected in 54 strains accounted for 84.38%, and the pattern of aadB + sulII was the most common genotype accounting for 23.44%. Therefore, the prevalence and antimicrobial susceptibility of P. multocida in goats should be controlled to protect their health and prevent spreading in husbandry environments.