Abstract
Milk contains many nutrients including carbohydrate, protein, fat, vitamins and minerals. Milk protein has high nutritional value because it contains all the essential amino acids. Considering the nutritive value, milk is widely consumed by the people as an ideal food. The biochemical changes in milk and milk products by microorganisms can be either desirable or undesirable. The safety of milk and milk products with respect to food borne diseases is of great concern around the world. Therefore, the present study was undertaken to determine the prevalence of Escherichia coli in milk and milk product with their antibiogram assay. A total of 150 milk and milk product (yogurt) samples were collected from Rajshahi Metropolitan area of Bangladesh and analyzed by cultural, staining and biochemical tests for the isolation and identification of E. coli. Antibiogram assay of all the isolates were done by disk diffusion method. The overall prevalence of E. coli was 20.0% in milk and milk product (yogurt) in Rajshahi Metropolitan area. The prevalence of E. coli was 26.0% and 34.0% in raw milk and in milk product (yogurt), respectively. E. coli was not detected in pasteurized milk in this study. In antibiogram assay, isolated E. coli showed 100.0%, 60.0%, 40.0%, 40.0%, 33.3%, 33.3%, 20.0%, and 10.0% resistance to penicillin, gentamycin, ampicillin, streptomycin, amoxycillin, sulfamethoxasole-trimethoprim, nalidixic acid, and ciprofloxacin, respectively. The isolates also showed 73.3%, 60.0%, 53.3%, 53.3%, 30.0%, 23.3%, and 20% sensitive to ciprofloxacin, nalidixic acid, sulfamethoxasole-trimethoprim, streptomycin, amoxycillin, ampicillin, and gentamycin, respectively. The findings of this experiment speculated that the use of ciprofloxacin and nalidixic acid may have the preference in the clinical control of milk borne E. coli infection in Bangladesh. J. Bio-Sci. 29(2): 81-91, 2021 (December)
Highlights
Milk is a fresh, clean and normal mammary secretion obtained by milking of one or more dairy animals for nourishment of their offspring (Rani and Maheshwari 2012)
The E. coli and other facultative anaerobes constitute about 0.1% of gut flora (Eckburg et al 2009) and oro-fecal transmission is the major route through which pathogenic strains of the bacterium cause disease
In the present study the overall prevalence of E. coli was 20.0% in milk and milk product in Rajshahi Metropolitan area of Bangladesh. This finding is in agreement with the findings of Annalselvamalar et al (2018) who reported that the prevalence of E. coli was 24.0% in milk and milk products in Chennai, India
Summary
Clean and normal mammary secretion obtained by milking of one or more dairy animals for nourishment of their offspring (Rani and Maheshwari 2012). The E. coli and other facultative anaerobes constitute about 0.1% of gut flora (Eckburg et al 2009) and oro-fecal transmission is the major route through which pathogenic strains of the bacterium cause disease. There are pathogenic strains of E. coli that when ingested, cause gastrointestinal illness in healthy humans and animals. The pathogenicity of E. coli strains is due to the presence of one or more virulence factors including invasiveness factors, heat labile and heat stable enterotoxins, verotxins, and colonization factors (Smith and Haibs 1967). A new generation of virulent and resistant type of E. coli is produced due to incomplete course of treatment of man and animal (Marshall et al 1990). The present study was conducted to isolate and identify E. coli from milk and milk product and antimicrobial sensitivity assay of this bacterium that has public health significance
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