Abstract
In this study, DES consisting of Choline hydroxide (ChOH) and Urea (Ur) was used to pretreat poplar under mild conditions (60 ℃). The 72 h saccharification efficiency of the pretreated samples for 10 min was significantly improved (69.97 %), which amounted to a 2.4-fold increase in sugar yield over the raw material (29.89 %). The remarkable enhancement could be correlated to the topochemical and morphological changes occurring in poplar cell walls, including the removal of lignin and hemicelluloses, decreased crystallinity and microstructural disruption. Noteworthy, X-ray diffraction analysis showed that cellulose I underwent a crystallographic transformation from cellulose I to cellulose II during DES pretreatment. Our study demonstrated that ChOH/Ur pretreatment was quite effective in deconstructing poplar cell walls and increasing the enzymatic hydrolysis efficiency of cellulose under mild conditions.
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