Abstract
In the current study, a simple cryopreservation method (desiccation) was applied to Corylus avellana L. callus. Accordingly, the effects of abscisic acid (ABA) concentration, pretreatment duration on MS medium containing ABA + 10% sucrose, and storage length in liquid nitrogen (LN) on the callus survival rate were investigated. Calli’s survival was assessed 8 weeks after exiting from LN. Callus survival after cryopreservation was significantly affected by the concentration and duration of the treatment with ABA-supplemented media. However, storage duration in LN did not have an impact on the callus survival rate. In the present study, the highest survival rate (45.79%) was obtained in both treatments of 20 days preculture on medium containing 2 mg l− 1 ABA following 2 h desiccation-one day storage in LN and 2.5 h desiccation-thirty days storage in LN.
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