Abstract
The aims of the experiment were to investigate the effect of pretreatment stress on the proliferation of anther-derived embryogenic callus, and its subsequent embryo regeneration in Swainsona formosa. Anthers were pretreated in starvation medium at cold (4 °C) temperature and cultured. Cultures were initially placed in darkness for four weeks, followed by 16-h photoperiod at 25 ± 1 °C. The stress pretreatment was found to enhance embryogenic callus formation on cultured anthers. Further, somatic embryos were successfully regenerated from embryogenic callus upon transferred onto regeneration medium supplemented with 1% sucrose and 4.63 µM kinetin, and later plantlets were obtained. Meanwhile, nonembryogenic callus continued to grow resulting in more callus mass without showing any embryoid initiation.
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