Pretreatment of forage legumes under moderate salinity with exogenous salicylic acid or spermidine

Antonio Pizolato Neto,Durvalina Maria Mathias Dos Santos,Ayza Eugênio Viana Camargos,Sônia Maria Raymundo Carregari,Sonia Marli Zingaretti,Priscila Lupino Gratão,Rita De Cássia Alves

doi:10.4025/actasciagron.v42i1.42809

Antonio Pizolato Neto, Durvalina Maria Mathias Dos Santos + Show 5 more

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https://doi.org/10.4025/actasciagron.v42i1.42809

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Abstract

The present study aims to determine whether exogenous salicylic acid (SA) or spermidine (Spd) has any protective effect against salt stress. Seeds were subjected to 0, 20, 40, and 60 mM NaCl with or without salicylic acid or spermidine (0.5 mM) for 10 days. The evaluated variables were germination rate, shoot and root dry masses, glycine betaine content, lipid peroxidation, and the activities of superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX). The data were subjected to Tukey’s test (p ≤ 0.05). There was a growth increase, especially in plant shoots. The reduction in lipid peroxidation, as indicated by lower malondialdehyde (MDA) levels, can be explained by an increase in antioxidant activity when SA and Spd were added. When compared to CAT and APX, SOD was the least responsive enzyme to the addition of both SA and Spd in salt-stressed plants. SA and Spd partially reduced the effects of moderate salt stress in both plant species; however, Spd addition had better results than SA in terms of suppressing oxidative stress. Lablab plants were more vigorous than pigeonpea plants.

Highlights

Saline soils are considered one of the greatest barriers to the growth and development of plants, representing one of the most worrying abiotic stresses for the scientific community. Pirasteh-Anosheh, Ranjbar, Pakniyat, and Emam, (2016) considered salinity to be most important abiotic stress and to be more important than drought because salt stress can occur anywhere, even when water resources are not limited
The alleviation of salinity stress using the exogenous application of several plant growth regulators such as salicylic acid (SA) and Spd has been of interest to researchers
We verified that only the SA application resulted in an increase in germination for both species under the lowest concentration of NaCl (20 mM) and that there was a decrease in germination under 60 mM NaCl for both species caused by the application of 0.5 mM SA and Spd when compared to the control

Summary

Introduction

Saline soils are considered one of the greatest barriers to the growth and development of plants, representing one of the most worrying abiotic stresses for the scientific community. Pirasteh-Anosheh, Ranjbar, Pakniyat, and Emam, (2016) considered salinity to be most important abiotic stress and to be more important than drought because salt stress can occur anywhere, even when water resources are not limited. Saline soils are considered one of the greatest barriers to the growth and development of plants, representing one of the most worrying abiotic stresses for the scientific community. The effects of NaCl on plants include osmotic stress, due to lower cellular hydric potential and ionic stress due to the cytotoxicity of the saline ions Na+ and Cl-, leading to a nutritional imbalance mainly in K, Ca, Mg, and as a consequence of both stresses (osmotic and ionic), the production of reactive oxygen species (ROS), which mainly consist of singlet oxygen (1O2), hydrogen peroxide (H2O2), superoxide anions (O-2), and hydroxyl radicals (OH), is triggered, which causes damage to living cells (Abogadallah, 2010; Munns & Gilliham, 2015; Pirasteh-Anosheh et al, 2016). According to Khan, Asgher, and Khan (2014), to counteract the

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