Abstract

The effect of different alkaline pretreatment conditions with sodium hydroxide (NaOH) on the recuperation of cellulosic fraction, lignin removal and ethanol production was evaluated through simultaneous saccharification and fermentation of elephant grass and king grass ((Pennisetum purpureum and Pennisetum hybridum). These types of grass are potential raw materials for bioethanol production from cellulosic fraction. Results obtained in ethanol production show that under pretreatment conditions of 120oC, 60 minutes, 2%(w/w) of NaOH and a liquid to solid ratio of 20 (w/w), the highest ethanol concentrations are obtained: 27.7 g/L and 26.1 g/L for king grass and elephant grass respectively, in 24 hours of fermentation. Furthermore, under the evaluated conditions it was observed that lignin removal was 88.4% for elephant grass and 94.0% for king grass. The detoxification stage eliminates inhibitors formed during pretreatment, which affects the hydrolysis and fermentation.

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