Pretectotectal pathway: An ultrastructural quantitative analysis in cats

Abstract

Both the pretectum (PT) and the superior colliculus (SC) play an important role in directing eye movements and in sensorimotor coupling. A reciprocal connection between the PT and the SC has been described, which suggests a strong interplay between these two structures. We injected the cat SC with retrograde tracers and examined the labeled pretectotectal (PTT) cells at the light and electron microscopic level. PTT cells were distributed mostly in the nucleus of the optic tract and 93.1% contained gamma amino butyric acid (GABA). We also observed that PTT cells are located outside of pretectal regions distinguished by dense retinal terminals and clusters of cells that contain calbindin. This suggests that the GABAergic PTT cells are distinct from the GABAergic pretectogeniculate cells that have been previously described as being distributed within these regions. Finally, to determine the synaptic targets of PTT terminals, we injected the PT with anterograde tracers and examined terminals labeled in the SC at the ultrastructural level. The labeled PTT terminals were beaded fibers that were distributed mainly within the stratum griseum superficiale (SGS) of the SC. Using postembedding immunocytochemistry, 94.5% were found to be GABAergic. The PTT terminals were mostly small in size and primarily contacted GABA-negative dendrites (88.1%) and in some cases somata (4.7%). The remainder terminated on GABAergic dendrites (7.2%). Our results suggest that the PTT cells constitute a separate population of GABAergic efferent cells in the PT, which may function to inhibit the activity of non-GABAergic SC efferent cells in the SGS.