Presumption of the Concentration of Several Constituents in the Culture Broth of Mouse-Mouse Hybridoma by Near Infrared Reflectance Spectroscopy

Abstract

For the control of animal cell culture, it is very important to be able to easily and rapidly measure the concentrations of glucose and glutamine as energy and carbon sources and of lactic acid and ammonia which are growth-inhibited products. Though several methods with the use of electrodes and enzymes have been developed for the detection of these compounds, the measurement procedures involved in these methods are not easy nor rapid. In the present study, near infrared spectroscopy (NIR) was applied in the presumption of the concentration of these nutrients and products in the culture broth. The correlation between the concentration obtained by the general method and that obtained by NIR was studied by multiple regression analysis. NIR values presumed by 5 wavelength-regression were in good agreement with those by the general method. The multiple correlation coefficients were over 0.98 in all the studied substances. The operation procedure involved in NIR is simple with the operation time required to predict the concentrations of these substances being only 5 min. The results of the present study suggest that NIR is a useful method for the control of animal cell culture.